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线粒体 Ca2 +保留容量检测和 Ca2 +触发的线粒体肿胀试验
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Neuroscience
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JoVE Journal Neuroscience
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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线粒体 Ca2 +保留容量检测和 Ca2 +触发的线粒体肿胀试验

DOI:
10.3791/56236-v

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05:53 min

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May 01, 2018

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, ,
1Department of Neurology,Qilu Hospital of Shandong University, 2Department of Neurology,Qingdao Municipal Hospital, 3Brain Research Institute,Qilu Hospital of Shandong University

Chapters

  • 00:04Title
  • 00:28Isolation of Mitochondria
  • 02:41Determination of Mitochondrial Ca2 Retention Capacity and Ca2-induced Mitochondrial Swelling
  • 04:33Results: The Measurement of Ca2 Retention Capacity and Ca2-induced Mitochondrial Swelling
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

本协议的目的是描述一种检查 ca2 +保留容量和 ca2 +触发的 SH-SY5Y 细胞线粒体肿胀的方法逐步。

Tags

Mitochondrial Calcium Retention CapacityCalcium-induced Mitochondrial SwellingMitochondrial DysfunctionEnergy MetabolismSH-SY5Y CellsMitochondrial IsolationProtease InhibitorCell HomogenizationDifferential CentrifugationMitochondrial Protein QuantificationBCA AssayCalcium Binding DyePotassium Chloride Media

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