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Biochemistry

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JoVE Journal Biochemistry
Um método alternativo de cultura para manter Hypomethylation genômico das células-tronco embrionárias Mouse usando MEK inibidor PD0325901 e vitamina C
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Um método alternativo de cultura para manter Hypomethylation genômico das células-tronco embrionárias Mouse usando MEK inibidor PD0325901 e vitamina C

Article DOI: 10.3791/56391
June 1st, 2018 •
1, 1, 1
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

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June 1st, 2018

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Descrevemos em detalhes dois protocolos de base química para cultivo de células-tronco embrionárias do mouse. Esse novo método utiliza mecanismos sinérgicos de promover oxidação mediada por Tet (vitamina C) e reprimindo a síntese de novo de 5-metilcitosina (por PD0325901) para manter o DNA hypomethylation e pluripotência das células-tronco embrionárias do mouse.

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