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基于同位素示踪实验结合的工作流研究多营养源的微生物代谢

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

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基于同位素示踪实验结合的工作流研究多营养源的微生物代谢

DOI:

10.3791/56393-v

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12:47 min

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January 22, 2018

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Audrey Bloem, Stephanie Rollero, Pauline Seguinot, Lucie Crépin, Marc Perez, Christian Picou, Carole Camarasa

¹UMR SPO, INRA, SupAgroM,Université de Montpellier, ²IWBT Stellenbosch University, ³PILI,Toulouse White Biotechnology

Chapters

00:05Title
01:23Fermentation and Sampling
03:52Quantification of Consumed Nitrogen Sources and Proteinogenic Amino Acids
05:38Measurement of Isotopic Enrichment of Proteinogenic Amino Acids
08:53Quantification and Isotopic Enrichment of Volatile Compounds and Calculations
10:21Results: Investigation of Microbial Metabolism of Multiple Nutrient Sources
11:38Conclusion

Summary

Automatic Translation

本议定书描述了一个实验过程, 定量和全面地研究多种营养来源的代谢。这一工作流程, 基于同位素示踪实验和分析程序的结合, 使被消耗的营养素的命运和由微生物综合的分子的新陈代谢的起源被确定。

Tags

Isotopic Tracer Experiments Microbial Metabolism Multiple Nutrient Sources Nitrogen Metabolism Yeast Fermentation Labeled Substrates Protein Distribution Metabolic Origin Environmental Conditions Reproducibility Physiological Stage Synthetic Medium Nitrogen Source Saccharomyces Cerevisiae Cell Population Fermentation Platform

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