Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Evan Bardot; Nikos Tzavaras; Deanna L. Benson; Nicole C. Dubois

doi:10.3791/56446

Journal

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Developmental Biology

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マウス胚における心臓前駆細胞集団の定量的ホールマウント免疫染色解析

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

マウス胚における心臓前駆細胞集団の定量的ホールマウント免疫染色解析

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9,574 Views

•

09:42 min

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October 12, 2017

DOI:

10.3791/56446-v

DOI:

10.3791/56446-v

•

09:42 min

October 12, 2017

•

3 Views

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core,Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience,Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

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Summary

ここでは、全体のマウント蛍光マウス初期胚のイメージに基づく定量的容積測定分析のためのプロトコルについて述べる.定性的と定量的開発時に心臓の構造を評価し、他の器官に広く適応可能があることを提案する強力な方法としてこのテクニックを紹介します。

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マウス胚における心臓前駆細胞集団の定量的ホールマウント免疫染色解析

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マウス胚における心臓前駆細胞集団の定量的ホール マウント免疫染色解析

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マウス胚における心臓前駆細胞集団の定量的ホールマウント免疫染色解析