Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Evan Bardot; Nikos Tzavaras; Deanna L. Benson; Nicole C. Dubois

doi:10.3791/56446

/

Análisis de la inmunofluorescencia de Whole-mount cuantitativa de las poblaciones progenitoras cardiacas en embriones de ratón

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Análisis de la inmunofluorescencia de Whole-mount cuantitativa de las poblaciones progenitoras cardiacas en embriones de ratón

Please note that all translations are automatically generated. Click here for the English version.

9,544 Views

•

09:42 min

•

October 12, 2017

DOI:

10.3791/56446-v

DOI:

10.3791/56446-v

•

09:42 min

October 12, 2017

•

3 Views

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core,Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience,Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

Transcript

Summary

Aquí, describimos un protocolo para montaje entero de la inmunofluorescencia y análisis volumétrico cuantitativo basado en imágenes de embriones de ratón de etapa temprana. Presentamos esta técnica como un potente enfoque cualitativa y cuantitativamente evaluar estructuras cardiacas durante el desarrollo, y proponer que sea ampliamente adaptable a otros sistemas del órgano.