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Journal
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Developmental Biology
/
小鼠胚胎心肌内祖群的定量全贴免疫荧光分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胚胎心肌内祖群的定量全贴免疫荧光分析
DOI:
10.3791/56446-v
•
09:42 min
•
October 12, 2017
•
Evan Bardot
2,3
,
Nikos Tzavaras
,
Deanna L. Benson
6
,
Nicole C. Dubois
2,3
1
Cell, Developmental, and Regenerative Biology Department
,
Icahn School of Medicine at Mount Sinai
,
2
Mindich Child Health and Development Institute
,
Icahn School of Medicine at Mount Sinai
,
3
Black Family Stem Cell Institute
,
Icahn School of Medicine at Mount Sinai
,
4
Microscopy Core
,
Icahn School of Medicine at Mount Sinai
,
5
Department of Neuroscience
,
Icahn School of Medicine at Mount Sinai
,
6
Friedman Brain Institute
,
Icahn School of Medicine at Mount Sinai
Chapters
00:05
Title
00:55
Cardiac Crescent Stage Embryo Harvest and Processing
02:45
Immunofluorescence Staining
03:57
Embryo Mounting and Confocal Imaging
05:28
Image Analysis and Quantitative 3D Modeling
07:49
Results: Representative Confocal Image Processing
08:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一个协议的全贴装免疫荧光和图像定量容积分析早期小鼠胚胎。我们提出这项技术是一个强有力的方法, 定性和定量评估心脏结构在发展过程中, 并提出它可能会广泛适用于其他器官系统。
Tags
Quantitative Whole-mount Immunofluorescence
Cardiac Progenitor Populations
Mouse Embryos
Heart Development
Embryonic Organogenesis
Volumetric Imaging
Automated Analysis
Early Embryogenesis
Organ Morphogenesis
Embryo Dissection
Paraformaldehyde Fixation
Immunofluorescence Staining
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