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Kvantitativ hele-mount Immunofluorescence analyse av Cardiac stamfar populasjoner i Mouse embryoer
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1Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, 4Microscopy Core,Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute,Icahn School of Medicine at Mount Sinai
Chapters
- 00:05Title
- 00:55Cardiac Crescent Stage Embryo Harvest and Processing
- 02:45Immunofluorescence Staining
- 03:57Embryo Mounting and Confocal Imaging
- 05:28Image Analysis and Quantitative 3D Modeling
- 07:49Results: Representative Confocal Image Processing
- 08:56Conclusion
Her beskriver vi en protokoll for hele mount immunofluorescence og bildebasert kvantitative volumetric analyse av tidlig stadium mouse embryoer. Vi presenterer denne teknikken som en kraftig tilnærming til kvalitativt og kvantitativt vurdere cardiac strukturer under utvikling, og foreslår at det kan være mye tilpasses andre organsystemer.
