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方法研究线虫线虫中固有的蛋白质聚集与年龄的变化

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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

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方法研究线虫线虫中固有的蛋白质聚集与年龄的变化

DOI:

10.3791/56464-v

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11:57 min

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November 26, 2017

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Nicole Groh², Ivan Gallotta, Marie C. Lechler², Chaolie Huang, Raimund Jung, Della C. David

¹Protein Aggregation and Aging,German Center for Neurodegenerative Diseases (DZNE), ²Graduate School of Cellular and Molecular Neuroscience,German Center for Neurodegenerative Diseases (DZNE)

Chapters

00:05Title
00:53Liquid Culture for Treatment with RNAi Starting at L1
02:40Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
06:14Disruption of Animals
06:58Isolation of SDS-insoluble Proteins for Mass Spectrometry
10:03Results: Reduced IIS Prevents Age-dependent Protein Aggregation
11:26Conclusion

Summary

Automatic Translation

本文所提出的方法的目的是在模型生物体的正常衰老过程中, 探索蛋白质的聚集性, 即线虫。该协议代表了一个强大的工具, 以研究高度不溶性的大骨料, 形成与年龄和确定如何改变 proteostasis 影响蛋白质聚集。

Tags

Caenorhabditis Elegans Protein Aggregation Aging Proteostasis Inherent Protein Aggregation Protein Insolubility Model Organism Gene Knockdown RNAi Worm Culture Worm Collection M9 Buffer Centrifugation Protein Solubility

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