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Journal
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Developmental Biology
/
RANKL 小鼠骨髓破骨细胞培养法研究 mTORC1 在破骨细胞形成中的作用
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JoVE Journal
Developmental Biology
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
Please note that all translations are automatically generated.
Click here for the English version.
RANKL 小鼠骨髓破骨细胞培养法研究 mTORC1 在破骨细胞形成中的作用
DOI:
10.3791/56468-v
•
09:37 min
•
March 15, 2018
•
Qinggang Dai*
1
,
Yujiao Han*
2
,
Furong Xie*
1
,
Xuhui Ma
,
Zhan Xu
,
Xiao Liu
,
Weiguo Zou
,
Jun Wang
1
Department of Pediatric Dentistry, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
,
National Clinical Research Center of Stomatology
,
2
State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology
,
University of Chinese Academy of Sciences
,
3
Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital
,
Shanghai Jiaotong University School of Medicine
Chapters
00:05
Title
00:59
Dissection and Isolation
03:27
Plating and Differentiation
05:41
Tartrate Resistant Acid Phosphatase Staining and Activity Quantification of Culture Medium
07:25
Results: Deletion of Raptor Impaired Osteoclast Formation
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿描述了一种从小鼠骨髓中分离和培养破骨细胞的
体外
的协议, 并研究雷帕霉素复合物1在破骨细胞形成中的作用。
Tags
Osteoclast
RANKL
MTORC1
Bone Marrow
In Vitro Culture
Osteoporosis
Cell Isolation
Cell Differentiation
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