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JoVE Journal
Developmental Biology

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Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
 

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Article doi: 10.3791/56557
November 3rd, 2017

Summary November 3rd, 2017

This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.

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