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Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
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Developmental Biology
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JoVE Journal Developmental Biology
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

DOI:
10.3791/56557-v

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07:38 min

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November 03, 2017

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,
1State Key Laboratory of Membrane Biology, Institute of Zoology,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

Chapters

  • 00:05Title
  • 01:06Aorta-gonad-mesonephros (AGM) Explant Culture and Colony Forming Units CFU) Analysis |
  • 04:24In Vivo AGM Transplantation
  • 06:08Results: Representative Effects of Fluoxetine on Hematopoietic Stem Cell (HSC) Development
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.

Tags

Keywords: Aorta-gonad-mesonephrosExplant CultureHematopoiesisHematopoietic Stem CellsHSC DevelopmentEmbryonicDissectionCollagenaseCell CultureM3434 Medium

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