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Developmental Biology
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主动脉-性腺-肾外植体培养体系在发育性造血中的应用
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Developmental Biology
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JoVE Journal
Developmental Biology
Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis
Please note that all translations are automatically generated.
Click here for the English version.
主动脉-性腺-肾外植体培养体系在发育性造血中的应用
DOI:
10.3791/56557-v
•
07:38 min
•
November 03, 2017
•
Junhua Lv
2
,
Feng Liu
2
1
State Key Laboratory of Membrane Biology, Institute of Zoology
,
Chinese Academy of Sciences
,
2
University of Chinese Academy of Sciences
Chapters
00:05
Title
01:06
Aorta-gonad-mesonephros (AGM) Explant Culture and Colony Forming Units CFU) Analysis |
04:24
In Vivo
AGM Transplantation
06:08
Results: Representative Effects of Fluoxetine on Hematopoietic Stem Cell (HSC) Development
07:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本议定书描述使用培养的主动脉-性腺-肾的表达分析, 菌落形成单位在文化和脾脏, 并长期重建, 以确定影响的调节因子和信号通路的造血干细胞发育。这已被证明是一个有效的系统研究造血干细胞生物学和功能。
Tags
Aorta-gonad-mesonephros
Explant Culture
Hematopoiesis
Hematopoietic Stem Cells
HSC Development
Embryonic
Dissection
Collagenase
Cell Culture
M3434 Medium
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