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Microscopia de imunofluorescência de γH2AX e 53BP1 para analisar a formação e reparação do DNA Double-strand Breaks
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
DOI:

10:47 min

November 03, 2017

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Chapters

  • 00:05Title
  • 00:52Material Preparation
  • 03:28Mononuclear Cells Isolation by Density Gradient Centrifugation
  • 05:26Cytospin Preparation, γH2AX and 53BP1 Immunofluorescence Staining, and Analysis
  • 08:46Results: Clearly Visible Foci and DNA Repair
  • 10:02Conclusion

Summary

Automatic Translation

Este manuscrito fornece um protocolo de análise de quebras de DNA dobro-costa pela microscopia de imunofluorescência de γH2AX e 53BP1.

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