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后半圆管法在新生小鼠内耳基因传递中的研究
JoVE Journal
Medicine
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JoVE Journal
Medicine
Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
Please note that all translations are automatically generated.
Click here for the English version.
后半圆管法在新生小鼠内耳基因传递中的研究
DOI:
10.3791/56648-v
•
03:52 min
•
March 02, 2018
•
Kevin Isgrig
,
Wade W. Chien
2
1
National Institute on Deafness and Other Communication Disorders
,
National Institutes of Health
,
2
Department of Otolaryngology-Head & Neck Surgery
,
Johns Hopkins School of Medicine
Chapters
00:05
Title
00:32
Procedure Setup
01:08
Surgical Approach
02:35
Results: Transduction of Utricular and Cochlear Hair Cells
03:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在本研究中, 我们描述了后半圆管方法作为一种可靠的方法, 为内耳基因分娩的新生小鼠。我们表明, 通过后半规管的基因传递能够灌注整个内耳。
Tags
Posterior Semicircular Canal
Inner Ear Gene Delivery
Neonatal Mouse
AAV8-whirlin
Gene Therapy
Hair Cell Transduction
Stereocilia Elongation
Surgical Dissection
Micro-injection
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