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Photoactivatable 激动剂对 T 细胞活化的时空控制

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Immunology and Infection

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JoVE Journal Immunology and Infection

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

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Photoactivatable 激动剂对 T 细胞活化的时空控制

DOI:

10.3791/56655-v

•

07:48 min

•

April 25, 2018

•

Elisa Sanchez², Morgan Huse

¹Immunology Program,Memorial Sloan-Kettering Cancer Center, ²Weill-Cornell Graduate School of Medical Sciences

Chapters

00:05Title
00:44Preparation of Stimulatory Glass Surfaces
02:48Image Acquisition
04:18Analysis of Fluorescence Intensity
05:10Analysis of Distance
05:58Results: TCR-induced DAG Accumulation and Centrosome Reorientation in 5C.C7 T Cells
07:09Conclusion

Summary

Automatic Translation

该协议描述了一种基于成像的方法, 激活 t 淋巴细胞使用 photoactivatable 肽 MHC, 使精确的时空控制 t 细胞活化。

Tags

Photoactivatable Peptide MHC T Cell Activation Spatial-temporal Control T Cell Signaling TIRF Microscopy CD4+ T Cells CD8+ T Cells Biotinylated Ligands Streptavidin Bio-PLL

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