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中性粒细胞衍生微粒的分离与鉴定功能研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
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中性粒细胞衍生微粒的分离与鉴定功能研究

DOI:
10.3791/56949-v

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06:56 min

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March 02, 2018

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, , ,
1Department of Pathology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Title
  • 00:55Murine Bone Marrow Polymorphonuclear (PMN) Cell Separation
  • 02:37Microparticle (MP) Isolation from Bone Marrow-derived Activated Murine PMNs
  • 03:45In Vivo PMN-derived Microparticle (MP) Wound Healing Assay
  • 04:38Results: Representative Effects of Isolated PMN-MPs in Epithelial Wound Healing
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了新的协议, 以分离和表征从人和小鼠中性粒细胞衍生的微粒。这些协议利用离心、流式细胞术和免疫印迹法技术对微粒含量进行分析, 可用于研究不同细胞类型的微粒在细胞功能中的作用。

Tags

Neutrophil-derived MicroparticlesCell-cell CommunicationDensity Gradient SeparationPolymorphonuclear CellsPMNMononuclear CellsCell-free SupernatantsUltra-centrifugationFunctional Studies

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