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胚胎干细胞的跨内细胞质量注射导致嵌合体率增高
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Biology
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JoVE Journal
Biology
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
Please note that all translations are automatically generated.
Click here for the English version.
胚胎干细胞的跨内细胞质量注射导致嵌合体率增高
DOI:
10.3791/56955-v
•
05:53 min
•
May 29, 2018
•
Gregory J. Scott
,
Artiom Gruzdev
,
Thomas B. Hagler
,
Manas K. Ray
1
Knock Out Mouse Core
,
NIEHS/NIH
Chapters
00:05
Title
00:44
Embryo Collection
02:01
Embryo Injection and Transfer
04:26
Results: Representative Post-embryonic Stem (ES) Cell Transfer Pregnancy, Survival, and Chimersim Rates
04:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以增加嵌合体生产不使用新设备。一个简单的方向改变的胚胎注射可以增加胚胎的数量, 并有可能缩短时间的生殖传播。
Tags
Embryonic Stem Cells
Chimera
Trans-inner Cell Mass Injection
Blastocyst
Microinjection
Cell Line
Cell Culture
Biologist
Graduate Student
Microscopy
Laboratory Techniques
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