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Cancer Research
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肿瘤组织中高通量芯片测序的染色质状态全基因组图谱集成平台
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
Please note that all translations are automatically generated.
Click here for the English version.
肿瘤组织中高通量芯片测序的染色质状态全基因组图谱集成平台
DOI:
10.3791/56972-v
•
10:41 min
•
April 05, 2018
•
Christopher Terranova
,
Ming Tang
,
Elias Orouji
,
Mayinuer Maitituoheti
,
Ayush Raman
,
Samirkumar Amin
,
Zhiyi Liu
,
Kunal Rai
1
Department of Genomic Medicine
,
University of Texas MD Anderson Cancer Center
,
2
The Jackson Laboratory for Genomic Medicine
Chapters
00:04
Title
00:31
Tissue/Cell Line Processing and Cross-linking
01:57
Tissue Lysis, Sonication, and Antibody Preparation
02:52
Chromatin Immunoprecipitation
03:40
Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
04:43
Purification and Quantification of Precipitated DNA
06:00
Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
07:53
Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
09:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个优化的高通量芯片排序协议和计算分析管道, 以确定从冷冻肿瘤组织和细胞系的全基因组的染色质状态模式。
Tags
Chromatin States
ChIP-sequencing
Cancer Epigenetics
Histone Modifications
Chromatin Fragments
Histone Antibodies
Protein G Magnetic Beads
ChIP Dilution Buffer
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