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Påvisning af en CDH1 sjældne udskrift Variant i frisk frosset gastrisk kræft væv af Chip-baserede digitale PCR
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
DOI:

09:16 min

February 05, 2018

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Chapters

  • 00:04Title
  • 01:03Preparing the PCR Reaction Mixes and the PCR Chip
  • 04:31Digital PCR (dPCR)
  • 05:30Chip Analysis
  • 07:05Results: Screening for CDH1a Expression in Tumor Tissue
  • 08:28Conclusion

Summary

Automatic Translation

Manuskriptet beskrives en chip-baserede digitale PCR assay til påvisning af en sjælden CDH1 udskrift variant (CDH1a) i frisk frosset normal og tumor væv fra patienter med mavekræft.

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