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Dissekere multi protein signalnettverk komplekser av resultatet av dette Complementation affinitet rensing (BiCAP)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
DOI:

06:45 min

June 15, 2018

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Chapters

  • 00:04Title
  • 00:45Plasmid Cloning
  • 02:54Cell Culture and Transfection
  • 03:41Harvesting Lysates
  • 04:31Affinity Purification and Preparation of BiCAP Eluant for Western Blotting
  • 05:22Results: BiCAP Method is a Powerful Technique to Specifically Purify and Characterize Interacting Proteins
  • 06:06Conclusion

Summary

Automatic Translation

Dette manuskriptet beskriver protokollen for resultatet av dette Complementation affinitet rensing (BiCAP). Denne romanen metoden forenkler bestemt isolasjon og nedstrøms proteomic karakteristikk av noen to samspill proteiner, mens unntatt FN-kompleksbundet personlige proteiner som komplekser dannet med konkurrerende bindende partnere.

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