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斑马鱼早期胚胎和肿瘤细胞电活动的可视化研究
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Developmental Biology
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JoVE Journal Developmental Biology
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
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斑马鱼早期胚胎和肿瘤细胞电活动的可视化研究

DOI:
10.3791/57330-v

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08:55 min

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April 25, 2018

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,
1Department of Comparative Pathobiology,Purdue University, 2Purdue University Center for Cancer Research, Purdue Institute for Inflammation, Immunology and Infectious Diseases (PI4D), Purdue Institute for Integrative Neuroscience (PIIN),Purdue University

Chapters

  • 00:05Title
  • 00:46Microinjection
  • 05:24Imaging
  • 06:52Results: Visualization of Embryonic Development, Movement, and Tumor Cells In Vivo
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们展示了创建一个细胞电压报告斑马鱼线的过程, 以可视化胚胎发育, 运动和鱼类肿瘤细胞在体内。

Tags

ZebrafishEmbryogenesisCellular Electrical ActivityTumorIn VivoReal-time ImagingTol2 TransposaseMRNA InjectionEmbryo CollectionAgarose Injection MoldMicroinjection

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