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Journal
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Immunology and Infection
/
检测梭菌感染后抗原特异性抗体应答的蛋白微阵
列测定方法
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following
Clostridium difficile
Infection
Please note that all translations are automatically generated.
Click here for the English version.
检测梭菌感染后抗原特异性抗体应答的蛋白微阵
列测定方法
DOI:
10.3791/57399-v
•
09:12 min
•
June 15, 2018
•
Ola H. Negm
2
,
Mohamed Hamed
2
,
Tanya M. Monaghan
1
Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen’s Medical Centre
,
University of Nottingham
,
2
Medical Microbiology and Immunology Department, Faculty of Medicine
,
Mansoura University
,
3
Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen’s Medical Centre
,
University of Nottingham
Chapters
00:04
Title
01:14
Preparing a Microarray Plate and Printing with a Contact Robot
03:06
Array Probing
05:19
Array Scanning
06:36
Results: Validation of Protein Microarray to Detect Isotype Specific Antibody Response to
Clostridium difficile
Toxins and Its Reproducibility
08:01
Conclusion
Summary
Automatic Translation
English (Original)
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本文介绍了一种简单的蛋白质芯片方法, 用于分析人体血清中高纯度
梭
菌抗原7丛细胞的体液免疫反应。该协议可推广用于测定多克隆静脉注射免疫球蛋白制剂中特异抗体的反应。
Tags
Protein Microarray
Clostridium Difficile
Antibody Response
Antigen
Immunotherapy
Multi-isotype
Strain-specific
Antigen Dilution
Microarray Printing
Slide Preparation
Arrayer Setup
Data Analysis
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