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Journal
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Biology
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气孔带皮: 一种改进的防护细胞样品制备方法
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Biology
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JoVE Journal
Biology
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation
Please note that all translations are automatically generated.
Click here for the English version.
气孔带皮: 一种改进的防护细胞样品制备方法
DOI:
10.3791/57422-v
•
08:52 min
•
July 15, 2018
•
Sheldon Lawrence II
2,3
,
Quiying Pang
3,4
,
Wenwen Kong
3
,
Sixue Chen
2,3,5
1
Plant Molecular and Cellular Biology Program
,
University of Florida
,
2
Department of Biology
,
University of Florida
,
3
Genetics Institute (UFGI)
,
University of Florida
,
4
Alkali Soil Natural Environmental Science Center
,
Northeast Forestry University
,
5
Interdisciplinary Center for Biotechnology Research (ICBR)
,
University of Florida
Chapters
00:02
Title
01:19
Preparation of Enriched Stomata
03:14
Protein Extraction and SDS-PAGE Separation
06:14
Results: Stomata Tape-Peel Allows for the Preparation of High Quality Enriched Stomata Suitable for Conducting Physiological, Biochemical and Molecular Assays
07:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了一种制备富含气孔保护细胞的方法, 用于生理学和其他生物研究。
Tags
Stomata
Guard Cells
Enrichment
Isolation
Transcriptomics
Proteomics
Metabolomics
Brassicaceae
Arabidopsis
Scotch Tape
Cell Wall Digestion
Phenol Extraction
Protein Precipitation
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