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Biology
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気孔テープ剥離: 細胞試料の調製法の改良
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Biology
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JoVE Journal
Biology
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation
Please note that all translations are automatically generated.
Click here for the English version.
気孔テープ剥離: 細胞試料の調製法の改良
DOI:
10.3791/57422-v
•
08:52 min
•
July 15, 2018
•
Sheldon Lawrence II
2,3
,
Quiying Pang
3,4
,
Wenwen Kong
3
,
Sixue Chen
2,3,5
1
Plant Molecular and Cellular Biology Program
,
University of Florida
,
2
Department of Biology
,
University of Florida
,
3
Genetics Institute (UFGI)
,
University of Florida
,
4
Alkali Soil Natural Environmental Science Center
,
Northeast Forestry University
,
5
Interdisciplinary Center for Biotechnology Research (ICBR)
,
University of Florida
Chapters
00:02
Title
01:19
Preparation of Enriched Stomata
03:14
Protein Extraction and SDS-PAGE Separation
06:14
Results: Stomata Tape-Peel Allows for the Preparation of High Quality Enriched Stomata Suitable for Conducting Physiological, Biochemical and Molecular Assays
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルでは、生理学的およびその他の生物学的研究に役立つ濃縮細胞を準備する方法について説明します。
Tags
Stomata
Guard Cells
Enrichment
Isolation
Transcriptomics
Proteomics
Metabolomics
Brassicaceae
Arabidopsis
Scotch Tape
Cell Wall Digestion
Phenol Extraction
Protein Precipitation
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