Journal
/
Biology
/
Magnetisk-aktivert celle sortering strategier for å isolere og rense Synovial Fluid-avledet Mesenchymal stamceller fra en kanin modell
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

Magnetisk-aktivert celle sortering strategier for å isolere og rense Synovial Fluid-avledet Mesenchymal stamceller fra en kanin modell

DOI:
10.3791/57466-v

•

10:20 min

•

August 10, 2018

•

, , , , , ,
1Postgraduate institution,Guangzhou Medical University, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, 3Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 4Department of Chemistry,Chinese University of Hong Kong, 5Shenzhen Kangning Hospital,Shenzhen Mental Health Center

Chapters

  • 00:04Title
  • 01:07Isolate and Culture the rbSF-MSCs
  • 03:21CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture
  • 05:01Magnetic Separation and Culture of te CD90+ rbSF-MSCs
  • 06:44Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikkelen presenterer en enkel og økonomisk protokoll for enkel isolasjon og rensing av mesenchymal stamceller fra New Zealand hvit kanin synovial væske.

Tags

Magnetic-activated Cell SortingMesenchymal Stem CellsSynovial FluidRabbit ModelIsolationPurificationFDA-approvedCell CultureCell SuspensionCentrifugationCell StrainerCell ColoniesCell Passaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Isolasjon og kultur av individuelle myofibers og deres satellitt celler fra voksne Skeletal Muscle

Induksjon og analyse av Epiteliale til Mesenchymale Transition

Nyreskade Mikroinjeksjon i Sebrafisk til Model Akutt nyreskade

Fluorescensaktivert cellesortering for isolering av sklerractiniske cellepopulasjoner

Storskala fremstilling av synovialvæske mesenkymale stamcelleavledede eksosomer ved 3D bioreaktorkultur

Isoler celletypespesifikke RNAer fra snapfrosne heterogene vevsprøver uten cellesortering

En enkel stasjonær filtreringsmetode for å isolere små ekstracellulære vesikler fra humane mesenkymale stamceller

Enkeltcellesortering av immunfenotypede mesenkymale stamceller fra humane eksfolierte løvtenner

Read Article