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小鼠初级少突胶质的快速特异磁分离
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Neuroscience
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JoVE Journal Neuroscience
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
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小鼠初级少突胶质的快速特异磁分离

DOI:
10.3791/57543-v

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09:32 min

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May 21, 2018

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, ,
1Program in Molecular and Cellular Biology,State University of New York Downstate Medical Center, 2Department of Neurology and Rehabilitation,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 00:40Mouse Brain Cortex Dissociation
  • 04:49O4+ Oligodendrocyte Isolation and Plating
  • 07:26Results: Representative Cell Surface Marker Expression During Mouse Oligodendrocyte Development
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了主鼠标少突胶质的磁隔离, 它允许对体外区域性的细胞进行快速和特定的隔离。

Tags

Immunomagnetic IsolationMouse Primary OligodendrocytesMyelinMyelinationNeural ScienceCell CultureDissociationNeurobasal A MediumBovine Growth SerumDNase IPipetting

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