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JoVE Journal
Immunology and Infection

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注射脂多糖对小鼠肠道屏障破坏后微生物源产物的模拟进入
 
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注射脂多糖对小鼠肠道屏障破坏后微生物源产物的模拟进入

Article DOI: 10.3791/57610-v 08:24 min May 2nd, 2018
May 2nd, 2018

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Summary

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本文提出了一种模拟肠道屏障破坏后细菌衍生化合物入口的协议。将低致死剂量的脂多糖系统地注入小鼠体内, 对24小时注射后进行监测。在脾、肝、结肠几个时间点上测定了促炎性细胞因子的表达。

Tags

免疫学和感染 问题 135 脂多糖 内毒素血症 结肠炎 炎症性肠病 屏障破坏 细胞因子
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