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Immunology and Infection

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JoVE Journal Immunology and Infection
Injektioner af LPS ind i musene at efterligne indgangen af mikrobielle-afledte produkter efter tarm barriere brud
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Injektioner af LPS ind i musene at efterligne indgangen af mikrobielle-afledte produkter efter tarm barriere brud

Article DOI: 10.3791/57610
May 2nd, 2018 •
1, 1, 1, 1, 1,2
1Department of Biomedicine, University of Basel, 2Division of Gastroenterology and Hepatology, University Hospital of Basel

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May 2nd, 2018

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Her præsenteres en protokol til at efterligne indgangen af bakteriel-afledte forbindelser efter tarm barriere brud. En lav subletale dosis af LPS blev sprøjtet systemisk ind i musene, som blev overvåget for 24 timer efter injektion. Udtryk af pro-inflammatoriske cytokiner var fast besluttet på flere tidspunkter i milten, leveren og kolon.

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