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Injeksjoner av lipopolysakkarid i mus å etterligne av mikrobielle avledede produkter etter Intestinal barriere brudd
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
DOI:

08:24 min

May 02, 2018

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Chapters

  • 00:05Title
  • 00:57Intraperitoneal Injection of LPS to Mice
  • 01:53Tissue Sampling for Ribonucleic Acid (RNA) Extraction and Homogenization
  • 03:22RNA Extraction from Tissue
  • 05:47Digestion of Remaining DNA
  • 06:51Results: LPS Injection Induces the Expression of Pro-inflammatory Cytokines in C57Bl/6 Mice
  • 07:43Conclusion

Summary

Automatic Translation

Her vises en protokoll å etterligne av bakteriell-avledet forbindelser etter intestinal barriere brudd. En lav sublethal dose av lipopolysakkarid ble injisert systemisk i mus, som var overvåket for 24 timer etter injeksjon. Uttrykk for pro-inflammatoriske cytokiner var fast bestemt på flere tidspunkt i milten og leveren kolon.

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