Journal
/
Biology
/
活体哺乳动物细胞器中单膜蛋白的多色定位显微术
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Please note that all translations are automatically generated. Click here for the English version.

活体哺乳动物细胞器中单膜蛋白的多色定位显微术

DOI:
10.3791/57690-v

•

11:06 min

•

June 30, 2018

•

, , , ,
1School of Biology,University of Osnabrück, 2Center of Cellular Nanoanalytics, Integrated Bioimaging Facility,University of Osnabrück, 3Department of Biology,WWU Münster

Chapters

  • 00:04Title
  • 00:54Physical Alignment of Image Splitter Generated Images
  • 02:25Alignment of Spectrally Separated Channels by Software Performing Spatial Transformation
  • 04:34Single Molecule Imaging of Mitochondrial Membrane Proteins
  • 08:32Results: Multi-color Localization of Mammalian Mitochondrial PINK1
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议的多颜色本地化的单膜蛋白在细胞器的活体细胞。要附加显影, 使用自贴标蛋白。蛋白质, 位于同一细胞器的不同的细胞膜车厢, 可以本地化的精度为 18 nm。

Tags

Multi-color Localization MicroscopySingle Membrane ProteinsOrganellesLive Mammalian CellsProtein DynamicsSpatiotemporal OrganizationMitochondrial Membrane ProteinsTransfectionSample PreparationMicroscope SetupFluorescent BeadsObjective LensImmersion OilTransmission LightLaserFluorescence ChannelsImage SplitterTIRF MicroscopyTransformation MatrixDual-color ImagesCalibrationUnit ConversionLocalization ManagerPoint Spread Function (PSF)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

长期使用宽视场显微镜的成像哺乳动物细胞

在活细胞荧光蛋白的双探头光学突出纯净的光转化的

在油脂介孔膜蛋白的结晶

纳米有限元分析：蛋白质定位的光激活定位显微镜和电子显微镜

使用机器人在油脂中间相高通量膜蛋白的结晶

在Live哺乳动物细胞中质粒复制多代荧光显微镜监测

活体显微镜成像的亚细胞结构的活体小鼠表达荧光蛋白

在造血干细胞和祖细胞利用共聚焦和多光子显微镜五荧光蛋白标记的活体克隆跟踪

高效的哺乳动物细胞表达和细胞外糖蛋白的结晶一步纯化

线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

Read Article