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Chapters
- 00:04Title
- 00:54Physical Alignment of Image Splitter Generated Images
- 02:25Alignment of Spectrally Separated Channels by Software Performing Spatial Transformation
- 04:34Single Molecule Imaging of Mitochondrial Membrane Proteins
- 08:32Results: Multi-color Localization of Mammalian Mitochondrial PINK1
- 10:15Conclusion
Här presenterar vi ett protokoll för flerfärgade lokalisering av enda membranproteiner i organeller av levande celler. Bifoga fluorophores genom används egen märkning proteiner. Proteiner, beläget i olika membran fack i samma organell, kan lokaliseras med en precision av ~ 18 nm.
Tags
Multi-color Localization MicroscopySingle Membrane ProteinsOrganellesLive Mammalian CellsProtein DynamicsSpatiotemporal OrganizationMitochondrial Membrane ProteinsTransfectionSample PreparationMicroscope SetupFluorescent BeadsObjective LensImmersion OilTransmission LightLaserFluorescence ChannelsImage SplitterTIRF MicroscopyTransformation MatrixDual-color ImagesCalibrationUnit ConversionLocalization ManagerPoint Spread Function (PSF)
