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Kwantitatieve celbiologie van neurodegeneratie in Drosophila door onbevooroordeelde analyse van Fluorescently Tagged eiwitten met behulp van ImageJ
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
DOI:

08:44 min

August 03, 2018

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Chapters

  • 00:04Title
  • 00:50Brain Dissection and Immunofluorescence Staining
  • 02:23Image Acquisition and Region of Interest (ROI) Selection
  • 04:19Preprocessing and Segmentation with H-Maxima Watershedding and Quantity, Area, and Intensity Quantification
  • 06:30Results: Representative Quantitative Analysis of Cell Biology of Neurodegeneration
  • 08:20Conclusion

Summary

Automatic Translation

Hebben we een eenvoudige en flexibele workflow om kwantitatieve gegevens extract van fluorescentie imaging-gebaseerde cel biologische studies van aggregatie van eiwitten en autophagic flux in het centrale zenuwstelsel van Drosophila modellen van neurodegeneratie.

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