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Humanized hilsen/SCID/IL2rγnull (hu-NSG) mus modell for HIV replikering og ventetid studier
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
DOI:

07:10 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
  • 01:59HSC Engraftment Validation
  • 03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
  • 05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
  • 06:26Conclusion

Summary

Automatic Translation

Denne protokollen gir en metode for å etablere humanized mus (hu-NSG) via intrahepatic injeksjon for menneskelige blodkreft stamceller i stråling-conditioned neonatal NSG mus. Hu-NSG musen er utsatt for HIV-smitte og kombinasjon antiretroviral terapi (handlevogn) og fungerer som en egnet patofysiologiske modell for HIV replikering og ventetid undersøkelser.

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