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Journal / Biochemistry / Affinitet rening av kloroplast Translocon…
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Biochemistry

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JoVE Journal Biochemistry
Affinitet rening av kloroplast Translocon proteinkomplex med taggen TAP
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Affinitet rening av kloroplast Translocon proteinkomplex med taggen TAP

Article DOI: 10.3791/58532-v • 07:01 min • November 1st, 2018
November 1st, 2018 •
1, 1, 2, 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

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Här presenterar vi en beprövad och testad protokoll för rening av kloroplast import proteinkomplex (TOC-TIC komplex) med TAP-taggen. One-step affinitet-isolering protokollet kan potentiellt tillämpas på något protein och används för att identifiera nya interaktion partners masspektrometri.

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Biokemi fråga 141 kloroplast TOC-TIC komplexa TAP tag TOC159 IgG bindande TEV proteas affinitet rening.
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