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Forberedelse af Poly(pentafluorophenyl acrylate) Functionalized SiO2 perler for Protein oprensning
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
DOI:

08:51 min

November 19, 2018

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Chapters

  • 00:04Title
  • 00:54Preparation of Poly(PFPA) Functionalized SiO2 Beads
  • 02:31Preparation of SiO2 Beads Grafted with PEG-Substituted Poly(PFPA)
  • 04:06Antibody Immobilization on Poly(PFPA) Grafted SiO2 Beads
  • 05:20Cell Lysis and Immunoprecipitation
  • 07:00Results: Analysis of Poly (pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
  • 08:09Conclusion

Summary

Automatic Translation

En protokol til forberedelse af poly (pentafluorophenyl acrylat) (poly(PFPA)) podet silica perler er præsenteret. Poly(PFPA) functionalized overflade er derefter immobiliseret med antistoffer og anvendt med succes til protein adskillelse gennem immunoprecipitation.

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