Journal
/
Neuroscience
/
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

DOI:
10.3791/59321-v

•

07:33 min

•

May 01, 2019

•

, , , , , , ,
1Department of Biology and Biotechnology Charles Darwin,Sapienza University of Rome, Italy, 2Center for Life Nano Science,Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology,The Rockefeller University, USA, 4Department of Physiology and Pharmacology,Sapienza University of Rome, Italy

Chapters

  • 00:04Title
  • 00:54Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation
  • 02:19Antibiotic Selection
  • 03:18Motor Neuron Differentiation
  • 05:12Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol allows rapid and efficient conversion of induced pluripotent stem cells into motor neurons with a spinal or cranial identity, by ectopic expression of transcription factors from inducible piggyBac vectors.

Tags

Human Induced Pluripotent Stem CellsIPSCsSpinal Motor NeuronsCranial Motor NeuronsPiggyBac VectorsMotor Neuron DiseasesAmyotrophic Lateral SclerosisCell DifferentiationTransfectionBlasticidin SelectionDoxycycline InductionRT-PCR

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

Read Article