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Journal
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Cancer Research
/
免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举
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Cancer Research
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JoVE Journal
Cancer Research
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Please note that all translations are automatically generated.
Click here for the English version.
免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举
DOI:
10.3791/59873-v
•
10:29 min
•
August 14, 2019
•
Jessica Garcia
2,3
,
David Barthelemy
2,3
,
Florence Geiguer
2
,
Julie Ballandier
2,3
,
Kathryn W. Li
,
Jean-Phillippe Aurel
,
Frédérique Le Breton
,
Claire Rodriguez-Lafrasse
,
Brigitte Manship
,
Sébastien Couraud
7
,
Léa Payen
2,3
1
Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud
,
Hospices Civils de Lyon
,
2
Circulating Cancer (CIRCAN) Program
,
Hospices Civils de Lyon Cancer Institute
,
3
University of Lyon, Claude Bernard University
,
Cancer Research Center of Lyon
,
4
Biolidics Limited
,
5
Institut de Pathologie Multisites des HCL-Site Sud
,
Hospices Civils de Lyon
,
6
Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital
,
Hospices Civils de Lyon Cancer Institute
,
7
EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty
,
Lyon 1 University
Chapters
00:04
Title
01:11
Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
03:10
Spiral Microfluidic Device CTC Enrichment
04:20
Immunofluorescence (IF) Staining
06:32
IF Image Acquisition and Analysis
08:07
Results: Representative CTC Characterization and Enumeration
09:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
循环肿瘤细胞(CTC)的表征是转化研究的一个热门话题。该协议描述了一种半自动免疫荧光(IF)测定,用于PD-L1表征和非小细胞肺癌(NSCLC)患者样本中CTC的枚举。
Tags
Circulating Tumor Cells
CTC
Non-small Cell Lung Cancer
PD-L1
Immunofluorescence
Microfluidic
Cell Characterization
Cell Enrichment
FISH
Transcriptomics
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