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1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology,The First Affiliated Hospital of Soochow University, 2Department of Pathology,University of Chicago, 3Department of Pathology,Brigham and Women’s Hospital–Harvard Medical School
Chapters
- 00:05Introduction
- 01:00Intestinal Organoid Isolation and Culture
- 04:18Flow Cytometry Analysis of EdU-Positive Cells in Enteroids
- 08:40Results: IL-22 Increases Enteroid Proliferation and Promotes Cell Death in Enteroids
- 09:46Conclusion
Il protocollo presentato utilizza la citometria di flusso per quantificare il numero di cellule proliferatie e morte negli enteroidi dei topi coltivati. Questo metodo è utile per valutare gli effetti del trattamento farmacologico sulla proliferazione e la sopravvivenza degli organiidi.
