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Journal
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Immunology and Infection
/
膣内HIV曝露とHIV感染治療に対するヒト化NOGマウス
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
Please note that all translations are automatically generated.
Click here for the English version.
膣内HIV曝露とHIV感染治療に対するヒト化NOGマウス
DOI:
10.3791/60723-v
•
08:15 min
•
January 31, 2020
•
Anna H. F. Andersen
2
,
Stine S. F. Nielsen
2
,
Rikke Olesen
2
,
Katharina Mack
,
Frederik Dagnæs-Hansen
,
Niels Uldbjerg
4
,
Lars Østergaard
2
,
Ole S. Søgaard
2
,
Paul W. Denton
2,5
,
Martin Tolstrup
2
1
Department of Clinical Medicine
,
Aarhus University
,
2
Department of Infectious Diseases
,
Aarhus University Hospital
,
3
Department of Biomedicine
,
Aarhus University
,
4
Department of Gynecology and Obstetrics
,
Aarhus University Hospital
,
5
Department of Biology
,
University of Nebraska at Omaha
Chapters
00:04
Title
00:50
Cord Blood CCR5Δ32 Genetic Variant Screening
01:50
Intravaginal HIV Exposure
03:20
RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
04:55
Application of Combination Antiretroviral Therapy (cART)-Containing Chow
05:42
Results: Representative HIV infection and Treatment DATA
07:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
幹細胞移植、膣内ヒト免疫不全ウイルス暴露、液滴デジタルPCR RNAを用いたヒト化免疫不全ウイルス感染NOGマウスモデルの生成と評価のためのプロトコルを開発しました。定量化。
Tags
Humanized NOG Mice
Intravaginal HIV Exposure
HIV Infection
Preclinical HIV Research
Viral Strains
HIV Interventions
CCR5 Variant
Viral RNA Quantification
Cord Blood Screening
CCR5 Delta 32
HIV Exposure Protocol
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