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Humaniserade NOG Möss för intravaginal HIV-exponering och behandling av HIV-infektion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
DOI:

08:15 min

January 31, 2020

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Chapters

  • 00:04Title
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

Summary

Automatic Translation

Vi har utvecklat ett protokoll för generering och utvärdering av en humaniserad och human immunbrist virusinfekterade NOG musmodell baserad på stamcellstransplantation, intravaginal human immunbrist virus exponering, och droplet digital PCR RNA Kvantifiering.

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