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Developmental Biology
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使用光明场显微镜定量拉瓦尔斑马鱼的肝脏大小
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用光明场显微镜定量拉瓦尔斑马鱼的肝脏大小
DOI:
10.3791/60744-v
•
07:35 min
•
February 02, 2020
•
Srishti Kotiyal*
1
,
Alexis Fulbright*
1
,
Liam K. O’Brien*
1
,
Kimberley J. Evason
1
Department of Pathology, Department of Oncological Sciences, and Huntsman Cancer Institute
,
University of Utah School of Medicine
Chapters
00:05
Introduction
00:49
Fixing Larvae and Dissecting Tissues Surrounding Liver
02:53
Imaging
04:05
Image Analysis
06:07
Results: Zebrafish Larvae
06:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们演示了一种量化幼虫斑马鱼肝脏大小的方法,提供了一种评估遗传和药理操作对肝脏生长和发育的影响的方法。
Tags
Liver Size
Larval Zebrafish
Brightfield Microscopy
Organ Quantification
Genetic Manipulation
Pharmacologic Manipulation
Liver Growth
Liver Development
Dissection
In Situ Hybridization
Confocal Imaging
Euthanasia
PBS
PFA
Skin Removal
Yolk Removal
Forceps
Glass Dish
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