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Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts
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JoVE Journal Biology
Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

DOI:
10.3791/60785-v

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05:56 min

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January 22, 2020

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, ,
1Zietchick Research Institute

Chapters

  • 00:04Title
  • 01:18Dissection and Tissue Homogenization
  • 02:20Bovine and Porcine LH ELISA Assay
  • 03:35Bovine and Porcine VEGF-A-ELISA Assay
  • 04:51Results: Linear Relationship of LH and VEGF
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for utilizing a cortical kidney extract preparation and total protein normalization to demonstrate the correlation between vascular endothelial growth factor and luteinizing hormone in the mammalian kidney.

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Keywords: Vascular Endothelial Growth FactorVEGFLuteinizing HormoneKidney Cortex ExtractsRenal PhysiologyAngiogenesisELISAProtein AnalysisSample Preparation

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