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西普库鲁斯核素体液的原位杂交

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Biology
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JoVE Journal Biology

In situ Hybridization for Sipunculus nudus Coelomic Fluid

Please note that all translations are automatically generated. Click here for the English version.

西普库鲁斯核素体液的原位杂交

DOI:

10.3791/61022-v

•

07:35 min

•

May 01, 2020

•

Wenhua Li, Mingrui Yuan, Yaqin Wu

¹Key Laboratory of Xiamen Marine and Gene Drugs, School of Biomedical Sciences,Huaqiao University

Chapters

00:00Introduction
00:44Collection of Coelomic Fluid
01:33In situ Hybridization: Day 1
03:20In situ Hybridization: Day 2
05:10In situ Hybridization: Day 3
06:17Results
07:15Conclusion

Summary

Automatic Translation

该协议描述了一种有效的原位杂交方法，用于检测西普库鲁斯核糖体流体中目标基因的mRNA表达水平和空间模式。

Tags

Keywords: In Situ Hybridization Sipunculus Nudus Coelomic Fluid MRNA Expression LncRNA Expression Gene Localization Fishery Resource RNA Probes Proteinase K PFA Fixation Wash Buffers Antibody Staining Alkaline Phosphatase BCIP-NBT Staining

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