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Ricostruzione delle firme di fluorescenza innata unicellulare mediante microscopia confocale
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Ricostruzione delle firme di fluorescenza innata unicellulare mediante microscopia confocale

Article DOI: 10.3791/61120-v • 07:29 min
May 27th, 2020 •
*1, *2, 1, 2,3, *2,3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Faculty of Life and Environmental Sciences, University of Tsukuba, 3Microbiology Research Center for Sustainability, University of Tsukuba
* These authors contributed equally

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May 27th, 2020

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Qui, viene presentato un protocollo per estrarre otticamente e catalogare le firme di fluorescenza cellulare innata (cioè l'autofluorescenza cellulare) da ogni singola cellula viva distribuita in uno spazio tridimensionale. Questo metodo è adatto per studiare la firma di fluorescenza innata di diversi sistemi biologici a una risoluzione unicellulare, comprese le cellule di batteri, funghi, lieviti, piante e animali.

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