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通过免疫荧光简单检测原发性西莉亚
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Simple Detection of Primary Cilia by Immunofluorescence
Please note that all translations are automatically generated.
Click here for the English version.
通过免疫荧光简单检测原发性西莉亚
DOI:
10.3791/61155-v
•
08:07 min
•
May 15, 2020
•
Alzbeta Filipova
,
Daniel Diaz Garcia
,
Josef Dvorak
,
Stanislav Filip
,
Marcela Jelicova
,
Zuzana Sinkorova
1
Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove
,
University of Defence
,
2
Department of Clinical Biochemistry and Diagnostics
,
University Hospital
,
3
Department of Oncology, Thomayer Hospital
,
Charles University
,
4
Department of Oncology and Radiotherapy, Faculty of Medicine
,
Charles University
Chapters
00:04
Introduction
00:40
Cell Culture for Immunocytochemistry Staining
03:10
Immunofluorescent Staining of Primary Cilia In Vitro
06:11
Results: Fluorescent Imaging of Primary Cilia in Fibroblasts
07:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
原发性西莉亚是与中环相关的细胞外结构。通过免疫荧光染色进行初级西莉亚检测是一个相对简单的过程,可产生极高质量的图像。在该协议中,表达原发性西莉亚的成纤维细胞被固定、免疫化,并在荧光或共合显微镜中成像。
Tags
Primary Cilia
Immunofluorescence
Cell Culture
Ciliation
Fluorescence
Basal Bodies
Fibroblasts
Trypsin EDTA
Paraformaldehyde
Gelatin
Hemocytometry
Cell Counting
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