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Journal
/
Immunology and Infection
/
香烟烟雾对肺
癫痫细胞
伪多多多细胞的影响研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Studying Effects of Cigarette Smoke on
Pseudomonas
Infection in Lung Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
香烟烟雾对肺
癫痫细胞
伪多多多细胞的影响研究
DOI:
10.3791/61163-v
•
09:15 min
•
May 11, 2020
•
Tiao Li
,
Chen Long
,
Kristen V. Fanning
,
Chunbin Zou
1
Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine
,
University of Pittsburgh School of Medicine
Chapters
00:04
Introduction
00:42
Cigarette Smoking Extract (CSE) Preparation
01:39
Pseudomonas
Culture
02:16
Cell Culture and CSE Treatment
03:33
Bacterial Infection and Gentamycin Treatment
04:24
Determination of Bacterial Concentration Using the Drop Plate Method
04:55
RT-qPCR Detection of Bacterial 16S rRNA
07:00
Results: CSE-Treatment Increases Bacterial Load
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里描述的是一个协议,研究香烟烟雾提取物如何影响肺上皮细胞的细菌殖民化。
Tags
Cigarette Smoke
Pseudomonas
Lung Epithelial Cells
Bacterial Load
Cell Culture
Smoke Extraction
Bacterial Infection
Cell Counting
Cell Suspension
Cell Preparation
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