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Induktion af diffus axonal hjerneskade hos rotter baseret på rotationsacceleration
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration
DOI:

06:14 min

May 09, 2020

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Chapters

  • 00:04Introduction
  • 00:45Preparing Rats for the Experimental Procedure
  • 01:11Procedure of Induction Diffuse Axonal Brain Injury
  • 01:54Measurement of Rotational Kinematics, Biomechanical Parameters
  • 02:32Evaluation of Neurological Severity Score after 48 hours
  • 03:08Brain Collection for Histological Examination after 48 hours
  • 04:02Immunohistochemical Staining
  • 04:16Results
  • 05:22Conclusion

Summary

Automatic Translation

Denne protokol validerer en pålidelig, nem at udføre og reproducerbare gnavere model af hjernen diffus axonal skade (DAI), der inducerer udbredt hvide stof skader uden kraniebrud eller kvæstelser.

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