JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
通过异位细胞系统中的蛋白质聚合测量跨细胞相互作用
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
Please note that all translations are automatically generated.
Click here for the English version.
通过异位细胞系统中的蛋白质聚合测量跨细胞相互作用
DOI:
10.3791/61237-v
•
04:47 min
•
May 22, 2020
•
Susana Restrepo
,
Samantha L. Schwartz
,
Matthew J. Kennedy
,
Jason Aoto
1
Department of Pharmacology
,
University of Colorado Denver School of Medicine
Chapters
00:04
Introduction
00:31
Cell Harvesting
02:12
Image Acquisition
02:50
Results: Point Mutation Enhances Binding
04:15
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里，我们提出了一个优化的协议，使用荧光显微镜快速和半定量测量异质细胞系统中跨体中的配体受体相互作用。
Tags
Protein Aggregation
Transcellular Interactions
Cell-based Assay
Trans Adhesion
Heterologous Cell System
HEK-293T Cells
EDTA
Hemocytometer
Fluorescence Imaging
Protein Of Interest
Ligand Of Interest
Synaptic Ligand
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测定在异种细胞表面的化学感应受体的表达
本地化的蛋白质三维神经干细胞培养：一个混合的可视化方法
人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导
窥视到社会相互作用的动力学测量打打架的影响
双突触诱发的星形胶质细胞和神经元的反应，在急性海马脑片电生理记录
一个轻量级的，耳机为基础的系统操作鸣禽听觉反馈
遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔
一<em>线虫</em>研究Amylopathy模型系统
使用胱天蛋白酶复分析，以确定细胞凋亡的下丘脑细胞模型
诱导星形胶质细胞受体的可塑性由神经元的放电频率操作
Read Article
