Three-Dimensional Motor Nerve Organoid Generation

Tatsuya Osaki; Siu Yu  A. Chow; Yui Nakanishi; Joel Hern&#225;ndez; Jiro Kawada; Teruo Fujii; Yoshiho Ikeuchi

doi:10.3791/61544

Journal

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Neuroscience

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Dreidimensionale Motornerve Organoid Generation

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Three-Dimensional Motor Nerve Organoid Generation

Dreidimensionale Motornerve Organoid Generation

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8,959 Views

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09:57 min

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September 24, 2020

DOI:

10.3791/61544-v

DOI:

10.3791/61544-v

•

09:57 min

September 24, 2020

•

34 Views

Tatsuya Osaki^1,2, Siu Yu A. Chow^1,2, Yui Nakanishi^1,2, Joel Hernández^1,3, Jiro Kawada⁴, Teruo Fujii¹, Yoshiho Ikeuchi^1,2

¹Institute of Industrial Science,The University of Tokyo, ²Department of Chemistry and Biotechnology, School of Engineering,The University of Tokyo, ³Faculty of Science and Engineering,Tecnologico de Monterrey, ⁴Jiksak Bioengineering, Inc.

Transcript

Summary

Dieses Protokoll bietet ein umfassendes Verfahren zur Herstellung des menschlichen iPS-Zell-abgeleiteten motorischen Nervenorganoids durch spontane Montage eines robusten Bündels von Axonen, das von einem Sphäroid in einem Gewebekulturchip erweitert wird.