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亚细胞结构的超分辨率活细胞成像
JoVE Journal
Biology
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JoVE Journal
Biology
Super-Resolution Live Cell Imaging of Subcellular Structures
Please note that all translations are automatically generated.
Click here for the English version.
亚细胞结构的超分辨率活细胞成像
DOI:
10.3791/61563-v
•
06:50 min
•
January 13, 2021
•
Rajesh Ranjan
,
Xin Chen
1
Department of Biology
,
The Johns Hopkins University
Chapters
00:04
Introduction
00:38
Glass Bottom Cell Culture Dish Preparation
01:10
Testes Dissection and Mounting
02:31
Live Cell In Situ Male Drosophila Germline Stem Cell (GSC) Imaging
04:15
Results: Representative Microtubule Time-Lapse Imaging
06:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍了一个在完整组织中进行超分辨率活细胞成像的协议。我们已经标准化了在其原生组织环境中成像高度敏感的成人干细胞群的条件。该技术涉及平衡时间和空间分辨率,以便直接观察活组织中的生物现象。
Tags
Super-resolution
Live Cell Imaging
Subcellular Structures
Fluorophore Probes
Physiological Condition
Cellular Process
Dialysis Membrane
Testes Dissection
Germline Stem Cell Imaging
Super Resolution Microscope
Transmitted Light
Laser Power
Photo Bleaching
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