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August 29, 2020
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We presenteren hier een methode die het gebruik van chemische epigenetische wissing combineert met mechanosensing-gerelateerde signalen om efficiënt pluripotente cellen van zoogdieren te genereren, zonder de noodzaak van gentransfectie of retrovirale vectoren. Deze strategie is daarom veelbelovend voor translationele geneeskunde en vertegenwoordigt een opmerkelijke vooruitgang in stamcelorganoïde technologie.
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Cite this Article
Pennarossa, G., Ledda, S., Arcuri, S., Gandolfi, F., Brevini, T. A. L. A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells. J. Vis. Exp. (162), e61655, doi:10.3791/61655 (2020).
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